{"id":73,"date":"2010-03-09T12:57:13","date_gmt":"2010-03-09T12:57:13","guid":{"rendered":"http:\/\/people.unica.it\/mvgebming\/?page_id=73"},"modified":"2010-05-19T08:36:37","modified_gmt":"2010-05-19T08:36:37","slug":"environmental-microbiology-laboratory","status":"publish","type":"page","link":"https:\/\/people.unica.it\/mvgebming\/environmental-microbiology-laboratory\/","title":{"rendered":"Environmental Microbiology Laboratory"},"content":{"rendered":"

Home <\/a>– Research <\/a>– Offices and Laboratories<\/a> – Laboratories Macchiareddu <\/a>– Environmental Microbiology Laboratory <\/span><\/p>\n

Specifications: <\/strong>Laboratory specialized in the analysis of environmental microbial communities and in the development of microbial biotechnologies for environmental and industrial applications.<\/span><\/p>\n

Equipment available<\/strong>: 2 Laminar flow biohazard hoods, 1 Incubator, 1 Cooling incubator, 1 CO2 Incubator, 3 Orbital shaker incubators, 1 Refrigerator, 2 Freezers (-20 \u00b0C), 1 Chemical hood, 1 Real Time PCR instrument, 2 PCR thermocyclers, 1 Tensiometer, 1 Refrigerated centrifuge, 1 Centrifuge, 1 Microcentrifuge, 1 FastPrep cell disrupter, 1 Spectrophotometer, 1 UV Transilluminator, 1 Photodocumentation system, 3 Microscopes, 1 Fluorescence microscope equipped with image analysis software, 3-liter fermentor, 1 Lyophiliser, Gel electrophoresis apparatus for nucleic acids and proteins, 1 Thermostatic bath, 1 Thermoblock, 1 Hot-air oven, 2 Vibrax shakers.<\/span><\/p>\n

\n\n\n\n
\"Laboratori<\/span><\/td>\n
\n<\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n
<\/strong><\/span><\/div>\n

Year of acquisition:<\/strong> 2004.<\/span><\/p>\n

Responsible: Dott.ssa Elena Tamburini<\/strong>.<\/span><\/p>\n

Ownership<\/strong>: Department of\u00a0 Biomedical Sciences and Technologies Section of\u00a0 General Microbiology and Virology & Microbial Biotechnologies, University of Cagliari<\/span><\/p>\n

Main applications:<\/strong><\/span><\/p>\n

\u2666 New strategies for the growth of \u201cmicroorganisms yet cultivable\u201d under lab conditions.<\/span><\/p>\n

\u2666 Typing and identification of microbial strains through molecular techniques \u00a0(ex. analysis of gene 16S rRNA, AFLP).<\/span><\/p>\n

\u2666 Study and monitoring of microbial communities and molecular techniques\u00a0 (ex. T-RFLP) and of standard microbiology to determine diversity and activities of microorganisms in natural environments.<\/span><\/p>\n

\u2666 Study and monitoring of microorganisms capable to degrade\/transform pollutants during bioremediation processes<\/span><\/p>\n

\u2666 High-throughput<\/em> techniques for isolation and screening<\/em> of microorganisms which produce surfactants and emulsifiers and evaluation of production efficiency and efficacy.<\/span><\/p>\n

\u2666 Optimization of culture conditions \u00a0for the production of microbial bioproducts bioprodotti through experimental techniques.<\/span><\/p>\n

\u2666 Cloning, expression in E. coli<\/em> and purification of enzyme of industrial interest (ex. pectinasi).<\/span><\/p>\n

\u2666 Analysis in silico <\/em>of nucleotide and amino acid sequences for phylogenetic and evolutionary studies.<\/span><\/p>\n","protected":false},"excerpt":{"rendered":"

Home – Research – Offices and Laboratories – Laboratories Macchiareddu – Environmental Microbiology Laboratory Specifications: Laboratory specialized in the analysis of environmental microbial communities and in the development of microbial biotechnologies for environmental and industrial applications. Equipment available: 2 Laminar flow biohazard hoods, 1 Incubator, 1 Cooling incubator, 1 CO2 Incubator, 3 Orbital shaker incubators, 1 Refrigerator, 2 Freezers (-20 \u00b0C), 1 Chemical hood, 1 Real Time PCR instrument, 2 PCR thermocyclers, 1 Tensiometer, 1 Refrigerated centrifuge, 1 Centrifuge, 1 Microcentrifuge, 1 FastPrep cell disrupter, 1 Spectrophotometer, 1 UV Transilluminator, 1 Photodocumentation system, 3 Microscopes, 1 Fluorescence microscope equipped with […]<\/a><\/p>\n","protected":false},"author":49,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-73","page","type-page","status-publish","hentry","category-1-id","post-seq-1","post-parity-odd","meta-position-corners","fix"],"_links":{"self":[{"href":"https:\/\/people.unica.it\/mvgebming\/wp-json\/wp\/v2\/pages\/73","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/people.unica.it\/mvgebming\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/people.unica.it\/mvgebming\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/people.unica.it\/mvgebming\/wp-json\/wp\/v2\/users\/49"}],"replies":[{"embeddable":true,"href":"https:\/\/people.unica.it\/mvgebming\/wp-json\/wp\/v2\/comments?post=73"}],"version-history":[{"count":4,"href":"https:\/\/people.unica.it\/mvgebming\/wp-json\/wp\/v2\/pages\/73\/revisions"}],"predecessor-version":[{"id":171,"href":"https:\/\/people.unica.it\/mvgebming\/wp-json\/wp\/v2\/pages\/73\/revisions\/171"}],"wp:attachment":[{"href":"https:\/\/people.unica.it\/mvgebming\/wp-json\/wp\/v2\/media?parent=73"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}