{"id":17,"date":"2010-03-09T10:18:10","date_gmt":"2010-03-09T10:18:10","guid":{"rendered":"http:\/\/people.unica.it\/mvgebming\/?page_id=17"},"modified":"2010-05-04T10:29:45","modified_gmt":"2010-05-04T10:29:45","slug":"research-lines","status":"publish","type":"page","link":"https:\/\/people.unica.it\/mvgebming\/research-lines\/","title":{"rendered":"Research Lines"},"content":{"rendered":"<p><a href=\"http:\/\/people.unica.it\/mvgebming\/\">Home <\/a>&#8211; <a href=\"http:\/\/people.unica.it\/mvgebming\/research\/\">Research<span style=\"color: #ffffff\"> <\/span><\/a><span style=\"color: #ffffff\">&#8211;<span style=\"color: #888888\"> <span style=\"color: #ffffff\">Research Lines<\/span><\/span><\/span><span style=\"color: #ffffff\"><br \/>\n<\/span><\/p>\n<p><span style=\"color: #ffffff\"><strong>Antiviral activity<\/strong><br \/>\nMain research lines in the antiviral chemotherapy field.<\/span><\/p>\n<p><span style=\"color: #ffffff\">\u2022 <strong>Rational development of antiviral agents<\/strong> through evaluation in cell-based assays of new derivatives whose design has been driven by SAR studies and, when appropriate, in silico prediction. .<br \/>\n\u2022 <strong>Target identification<\/strong> through time of addition assays, expression and purification of putative target enzymes and validation through IC50 determination in known or innovative enzyme assays. .<br \/>\n\u2022 Definition of the <strong>molecular bases of drug resistance<\/strong> through in vitro selection of mutants, definition of their cross-resistance patterns, genome sequencing to identify mutations responsible for resistance and localization of mutations in the 3D target enzyme structure and evaluation of mechanistic consequences.<br \/>\n\u2022 <strong>In silico studies<\/strong> through molecular docking and automated docking methods for studying the interaction of drugs with putative binding sites; molecular dynamics (MD) simulations to calculate free binding energies; modeling of drug-enzyme interactions for in silico prediction of IC50 and design of new molecules, using advanced simulation software.<\/span><\/p>\n<p><span style=\"color: #ffffff\"><strong>Microbial biotechnologies<\/strong><\/span><\/p>\n<p><span style=\"color: #ffffff\">Main research lines in <span style=\"text-decoration: underline\">industrial and environmental biotechnologies<\/span>.<\/span><\/p>\n<p><span style=\"color: #ffffff\">\u2022 Analysis and monitoring of microbial communities responsible for the degradation and transformation of pollutants during bioremediation and phytoremediation of soils and waters contaminated by heavy metals and hydrocarbons.<\/span><\/p>\n<p><span style=\"color: #ffffff\">\u2022\u00a0 Analysis of microbial diversity in natural environments for the development of biotechnological processes for the production of biosurfactants and bioemulsifiers.<\/span><\/p>\n<p><span style=\"color: #ffffff\">\u2022 In vitro evaluation of the antibacterial and antifungal activities of synthetic and natural compounds.<\/span><\/p>\n<p><span style=\"color: #ffffff\">\u2022 Study of pectinolitic bacteria and pectinases for applications in textile industry.<\/span><\/p>\n<p><span style=\"color: #ffffff\">More recent research lines on <span style=\"text-decoration: underline\">other topics<\/span> are the following:<\/span><\/p>\n<p><span style=\"color: #ffffff\">\u2022 Relationships between <strong>cholesterol homeostasis and<\/strong><em><strong> protein misfolding\/aggregation<\/strong><\/em> in Prion models in vitro and Alzheimer patients.<\/span><\/p>\n<p><span style=\"color: #ffffff\">\u2022 Development of anti-<em>protein misfolding\/aggregation <\/em>compounds and study of their mode of action.<\/span><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Home &#8211; Research &#8211; Research Lines Antiviral activity Main research lines in the antiviral chemotherapy field. \u2022 Rational development of antiviral agents through evaluation in cell-based assays of new derivatives whose design has been driven by SAR studies and, when appropriate, in silico prediction. . \u2022 Target identification through time of addition assays, expression and purification of putative target enzymes and validation through IC50 determination in known or innovative enzyme assays. . \u2022 Definition of the molecular bases of drug resistance through in vitro selection of mutants, definition of their cross-resistance patterns, genome sequencing to identify mutations responsible for resistance <a href='https:\/\/people.unica.it\/mvgebming\/research-lines\/' class='excerpt-more'>[&#8230;]<\/a><\/p>\n","protected":false},"author":49,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-17","page","type-page","status-publish","hentry","category-1-id","post-seq-1","post-parity-odd","meta-position-corners","fix"],"_links":{"self":[{"href":"https:\/\/people.unica.it\/mvgebming\/wp-json\/wp\/v2\/pages\/17","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/people.unica.it\/mvgebming\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/people.unica.it\/mvgebming\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/people.unica.it\/mvgebming\/wp-json\/wp\/v2\/users\/49"}],"replies":[{"embeddable":true,"href":"https:\/\/people.unica.it\/mvgebming\/wp-json\/wp\/v2\/comments?post=17"}],"version-history":[{"count":4,"href":"https:\/\/people.unica.it\/mvgebming\/wp-json\/wp\/v2\/pages\/17\/revisions"}],"predecessor-version":[{"id":147,"href":"https:\/\/people.unica.it\/mvgebming\/wp-json\/wp\/v2\/pages\/17\/revisions\/147"}],"wp:attachment":[{"href":"https:\/\/people.unica.it\/mvgebming\/wp-json\/wp\/v2\/media?parent=17"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}