BL2 (Biosafety Level 2) Bacteriology Laboratory

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Specifications: Laboratory specialized in the cultivation of laboratory and clinical strains of biosafety level 2 bacteria and fungi.

Equipment available: 2 Laminar flow biohazard hoods, 2 Incubators, 2 CO2 Incubators, 2 Orbital Shaker incubators, 1 Refrigerator, 1 Centrifuge, 1 Spectrophotometer, 4 Microscopes, 1 Thermostatic Bath, Filtration apparatus.

Year of acquisition: 2004.

Responsable: Dott.ssa Elena Tamburini

Ownership: Department of  Biomedical Sciences and Technologies Section of  General Microbiology and Virology & Microbial Biotechnologies, University of Cagliari

Main applications:

♦ Evaluation of antibacterial and antifungal activities of natural and synthetic compounds against laboratory and clinical strains:

–             Gram-positive bacteria: es. Staphylococcus aureus, Enterococcus faecalis

–             Gram-negative bacteria: es. Escherichia coli, Pseudomonas aeruginosa

–             Fungi: es. Candida albicans, Aspergillus fumigatus

–             Microbial biofilms

♦ Biocide activity:

–             Bactericide: Staphylococcus aureus, Pseudomonas aeruginosa

–             Fungicide: Candida albicans, Aspergillus niger

–             Sporicide: Bacillus cereus, Bacillus subtilis

–             Mycobactericide: Mycobacterium avium, Mycobacterium terrae