{"id":833,"date":"2010-03-01T15:20:32","date_gmt":"2010-03-01T13:20:32","guid":{"rendered":"http:\/\/people.unica.it\/mvgebm\/?page_id=833"},"modified":"2010-03-02T15:26:57","modified_gmt":"2010-03-02T13:26:57","slug":"molecular-biology-laboratory","status":"publish","type":"page","link":"https:\/\/people.unica.it\/mvgebm\/molecular-biology-laboratory\/","title":{"rendered":"Molecular Biology Laboratory"},"content":{"rendered":"

Home <\/a>– Research <\/a>– Offices and Laboratories<\/a> – Laboratorie Monserrato<\/a><\/a><\/span> – Molecular Biology Laboratory<\/span><\/p>\n

Specifications: <\/strong>Lab. specialized in molecular virology and viral enzymology.<\/span><\/p>\n

Equipment available<\/strong>: 1 Laminar flow biohazard hood, 2 Refrigerators (+4\/-20 \u00b0C) and 1 Freezers (-20 \u00b0C), 1 Beta counter, 1 Transilluminator, 1 Spectrophotometer, Gel electrophoresis apparatus for nucleic acids and proteins, 1 Thermostatic Bath, 1 Termoblock.<\/span><\/p>\n

\n\n\n\n
\"Laboratori<\/span><\/td>\n\"\"<\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n
<\/strong><\/span><\/div>\n

Year of acquisition:<\/strong> 1999<\/span><\/p>\n

Responsibles: Dr. Sylvain Blois<\/strong><\/span><\/p>\n

Ownership<\/strong>: Department of\u00a0 Biomedical Sciences and Technologies Section of\u00a0 General Microbiology and Virology & Microbial Biotechnologies, University of Cagliari<\/span><\/p>\n

Main applications:<\/strong><\/span><\/p>\n

\u2666\u00a0\u00a0 Real-time PCR analysis of HIV-1 replication in ex vivo target cells.<\/span><\/p>\n

\u2666\u00a0\u00a0 Nucleic acid extraction, RT-PCR and PCR for amplification and sequencing of wild type and drug-resistant viruses for identification of mutations involved in the acquisition of drug-resistance to nucleoside and non-nucleoside inhibitors of HIV-1 and other RNA viruses.<\/span><\/p>\n

\u2666\u00a0\u00a0 Cloning and expression in Escherichia coli of viral genes coding for enzymes target of selective antiviral chemotherapy (i.e. RNA polymerases).<\/span><\/p>\n

\u2666\u00a0\u00a0 Cloning of site-directed mutated genes coding for target enzymes in order to define the role of sites potentially involved in drug-resistance.<\/span><\/p>\n

\u2666\u00a0\u00a0 Expression and purification of viral wild type enzymes and mutants to be used in in vitro<\/em> enzymatic assays to evaluate antiviral inhibitors activity.<\/span><\/p>\n","protected":false},"excerpt":{"rendered":"

Home – Research – Offices and Laboratories – Laboratorie Monserrato – Molecular Biology Laboratory Specifications: Lab. specialized in molecular virology and viral enzymology. Equipment available: 1 Laminar flow biohazard hood, 2 Refrigerators (+4\/-20 \u00b0C) and 1 Freezers (-20 \u00b0C), 1 Beta counter, 1 Transilluminator, 1 Spectrophotometer, Gel electrophoresis apparatus for nucleic acids and proteins, 1 Thermostatic Bath, 1 Termoblock. Year of acquisition: 1999 Responsibles: Dr. Sylvain Blois Ownership: Department of\u00a0 Biomedical Sciences and Technologies Section of\u00a0 General Microbiology and Virology & Microbial Biotechnologies, University of Cagliari Main applications: \u2666\u00a0\u00a0 Real-time PCR analysis of HIV-1 replication in ex vivo target cells. […]<\/a><\/p>\n","protected":false},"author":49,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"open","template":"","meta":{"footnotes":""},"class_list":["post-833","page","type-page","status-publish","hentry","category-1-id","post-seq-1","post-parity-odd","meta-position-corners","fix"],"_links":{"self":[{"href":"https:\/\/people.unica.it\/mvgebm\/wp-json\/wp\/v2\/pages\/833","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/people.unica.it\/mvgebm\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/people.unica.it\/mvgebm\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/people.unica.it\/mvgebm\/wp-json\/wp\/v2\/users\/49"}],"replies":[{"embeddable":true,"href":"https:\/\/people.unica.it\/mvgebm\/wp-json\/wp\/v2\/comments?post=833"}],"version-history":[{"count":5,"href":"https:\/\/people.unica.it\/mvgebm\/wp-json\/wp\/v2\/pages\/833\/revisions"}],"predecessor-version":[{"id":1029,"href":"https:\/\/people.unica.it\/mvgebm\/wp-json\/wp\/v2\/pages\/833\/revisions\/1029"}],"wp:attachment":[{"href":"https:\/\/people.unica.it\/mvgebm\/wp-json\/wp\/v2\/media?parent=833"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}