{"id":654,"date":"2010-02-15T13:42:12","date_gmt":"2010-02-15T11:42:12","guid":{"rendered":"http:\/\/people.unica.it\/mvgebm\/?page_id=654"},"modified":"2010-03-01T14:40:31","modified_gmt":"2010-03-01T12:40:31","slug":"research-lines","status":"publish","type":"page","link":"https:\/\/people.unica.it\/mvgebm\/research-lines\/","title":{"rendered":"Research Lines"},"content":{"rendered":"<p><span style=\"color: #ffffff\"><a href=\"http:\/\/people.unica.it\/mvgebm\">Home <\/a>&#8211; <a href=\"http:\/\/people.unica.it\/mvgebm\/research\/\">Research <\/a>&#8211; Research Lines<\/span><\/p>\n<p><span style=\"color: #ffffff\">Main research lines in the antiviral chemotherapy field include the following.<\/span><\/p>\n<ul>\n<li><span style=\"color: #ffffff\"><strong>Rational development of antiviral agents<\/strong> through evaluation in cell-based assays of new derivatives whose design has been driven by SAR studies and, when appropriate, in silico prediction. .<\/span><\/li>\n<li><span style=\"color: #ffffff\"><strong>Target identification<\/strong> through time of addition assays, expression and purification of putative target enzymes and validation through IC50 determination in known or innovative enzyme assays. .<\/span><\/li>\n<li><span style=\"color: #ffffff\">Definition of the <strong>molecular bases of drug resistance<\/strong> through in vitro selection of mutants, definition of their cross-resistance patterns, genome sequencing to identify mutations responsible for resistance and localization of mutations in the 3D target enzyme structure and evaluation of mechanistic consequences.<\/span><\/li>\n<li><span style=\"color: #ffffff\"><strong>In silico studies<\/strong> through molecular docking and automated docking methods for studying the interaction of drugs with putative binding sites; molecular dynamics (MD) simulations to calculate free binding energies; modeling of drug-enzyme interactions for in silico prediction of IC50 and design of new molecules, using advanced simulation software.<\/span><\/li>\n<\/ul>\n<p><span style=\"color: #ffffff\">Main research lines in <span style=\"text-decoration: underline\">biotechnologies applied to industrial and environmental fields<\/span> include the following.<\/span><\/p>\n<p><span style=\"color: #ffffff\">&#8211; Roles of microbial communities in <strong>bioremediation<\/strong> of heavy metal- and hydrocarbon-contaminated soils and ground waters.<\/span><\/p>\n<p><span style=\"color: #ffffff\">&#8211; Monitoring of pollutant <strong>degrading\/transforming microorganisms<\/strong> during bioremediation.<\/span><\/p>\n<p><span style=\"color: #ffffff\">&#8211; Study of microbial diversity and its exploitation as a source of novel microorganisms and surface active compounds (<strong>surfactants and emulsifiers<\/strong>) for biotechnological applications.<\/span><\/p>\n<p><span style=\"color: #ffffff\">&#8211; Development of detergents and disinfectants.<\/span><\/p>\n<p><span style=\"color: #ffffff\">&#8211; Study of plant-associated bacteria in phyto-remediation of heavy metal-contaminated soils.<\/span><\/p>\n<p><span style=\"color: #ffffff\">&#8211; Study of pectinolytic bacteria and pectinase enzymes for application in textile industry.<\/span><\/p>\n<p><span style=\"color: #ffffff\">More recent research lines on <span style=\"text-decoration: underline\">other topics<\/span> are the following:<\/span><\/p>\n<p><span style=\"color: #ffffff\">&#8211; Relationships between <strong>cholesterol homeostasis and <em>protein misfolding\/aggregation<\/em><\/strong> in Prion models in vitro and Alzheimer patients.<\/span><\/p>\n<p><span style=\"color: #ffffff\">&#8211; Development of anti-<em>protein misfolding\/aggregation<\/em> compounds and study of their mode of action.<\/span><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Home &#8211; Research &#8211; Research Lines Main research lines in the antiviral chemotherapy field include the following. Rational development of antiviral agents through evaluation in cell-based assays of new derivatives whose design has been driven by SAR studies and, when appropriate, in silico prediction. . Target identification through time of addition assays, expression and purification of putative target enzymes and validation through IC50 determination in known or innovative enzyme assays. . Definition of the molecular bases of drug resistance through in vitro selection of mutants, definition of their cross-resistance patterns, genome sequencing to identify mutations responsible for resistance and localization <a href='https:\/\/people.unica.it\/mvgebm\/research-lines\/' class='excerpt-more'>[&#8230;]<\/a><\/p>\n","protected":false},"author":49,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"open","template":"","meta":{"footnotes":""},"class_list":["post-654","page","type-page","status-publish","hentry","category-1-id","post-seq-1","post-parity-odd","meta-position-corners","fix"],"_links":{"self":[{"href":"https:\/\/people.unica.it\/mvgebm\/wp-json\/wp\/v2\/pages\/654","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/people.unica.it\/mvgebm\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/people.unica.it\/mvgebm\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/people.unica.it\/mvgebm\/wp-json\/wp\/v2\/users\/49"}],"replies":[{"embeddable":true,"href":"https:\/\/people.unica.it\/mvgebm\/wp-json\/wp\/v2\/comments?post=654"}],"version-history":[{"count":5,"href":"https:\/\/people.unica.it\/mvgebm\/wp-json\/wp\/v2\/pages\/654\/revisions"}],"predecessor-version":[{"id":803,"href":"https:\/\/people.unica.it\/mvgebm\/wp-json\/wp\/v2\/pages\/654\/revisions\/803"}],"wp:attachment":[{"href":"https:\/\/people.unica.it\/mvgebm\/wp-json\/wp\/v2\/media?parent=654"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}