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Titolo: Selective gamma-hydroxybutyric acid receptor ligands increase extracellular glutamate in hippocampus, but fail to activate G protein and to produce the sedative/hypnotic effect of gamma-hydroxybutyric acid
Autori: 
Data di pubblicazione: 2003
Rivista: 
JOURNAL OF NEUROCHEMISTRY  
Abstract: Two gamma-hydroxybutyric acid (GHB) analogues, trans<x>-gamma-hydroxycrotonic acid (t-HCA) and gamma-(p-methoxybenzyl)-gamma-hydroxybutyric acid (NCS-435) displaced [H-3]GHB from GHB receptors with the same affinity as GHB but, unlike GHB, failed to displace [H-3]baclofen from GABA(B) receptors. The effect of the GHB analogues, GHB and baclofen, on G protein activity and hippocampal extracellular glutamate levels was compared. While GHB and baclofen stimulated 5'-O-(3-[S-35]thiotriphospate) [S-35]GTPgammaS binding both in cortex homogenate and cortical slices, t-HCA and NCS-435 were ineffective up to 1 mm concentration. GHB and baclofen effect was suppressed by the GABA(B) antagonist CGP 35348 but not by the GHB receptor antagonist NCS-382. Perfused into rat hippocampus, 500 nm and 1 mm GHB increased and decreased extracellular glutamate levels, respectively. GHB stimulation was suppressed by NCS-382, while GHB inhibition by CGP 35348. t-HCA and NCS-435 (0.1-1000 mum) locally perfused into hippocampus increased extracellular glutamate; this effect was inhibited by NCS-382 (10 mum) but not by CGP 35348 (500 mum). The results indicate that GHB-induced G protein activation and reduction of glutamate levels are GABA(B)-mediated effects, while the increase of glutamate levels is a GHB-mediated effect. Neither t-HCA nor NCS-435 reproduced GHB sedative/hypnotic effect in mice, confirming that this effect is GABA(B)-mediated. The GHB analogues constitute important tools for understanding the physiological role of endogenous GHB and its receptor.
Handle: http://hdl.handle.net/11584/4478
Tipologia:1.1 Articolo in rivista

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