Prodotti della ricerca

 
Titolo: DEXAMETHASONE INHIBITS INDUCTION OF LIVER-TUMOR NECROSIS FACTOR-ALPHA MESSENGER-RNA AND LIVER GROWTH INDUCED BY LEAD NITRATE AND ETHYLENE DIBROMIDE
Autori: 
Data di pubblicazione: 1994
Rivista: 
THE AMERICAN JOURNAL OF PATHOLOGY  
Abstract: We have recently demonstrated that a single injection of the mitogen lend nitrate to rats induced a rapid increase of tumor necrosis factor-alpha (TNF-alpha) mRNA in the liver and suggested that this cytokine may be involved in triggering hepatocyte proliferation in this model of direct hyperplasia. In this study, we examined whether a similar induction of river TNF-alpha mRNA could be observed preceding the onset of hepatocyte proliferation induced by ethylene dibromide, another hepatocyte mitogen In addition, we used dexamethasone, a well known inhibitor of TNF-alpha production, to determine whether its administration could suppress hepatocyte proliferation induced by lead nitrate and ethylene dibromide. A single intragastric administration of ethylene dibromide (100 mg/kg) to male Wistar rats enhanced liver TNF-alpha mRNA after 4 and 7 hours, which then returned to control levels by 24 hours. TNF-alpha mRNA was detectable only in a nonparenchymal cell fraction of the liver. Pretreatment of rats with a single dose of dexamethasone (2 mg/kg) 60 minutes before lead nitrate (100 mu mol/kg) or ethylene dibromide completely abolished the increased levels of liver TNF-alpha mRNA induced by these agents. Inhibition by dexamethasone of TNF-alpha mRNA tons associated with an inhibition of liver cell proliferation induced by these mitogens, as measured by [H-3]thymidine incorporation into hepatic DNA, mitotic index, and DNA content. These results further support the hypothesis that TNF-alpha may be involved in triggering hepatocyte proliferation induced by primary mitogens.
Handle: http://hdl.handle.net/11584/91140
Tipologia:1.1 Articolo in rivista

File in questo prodotto:
Non ci sono file associati a questo prodotto.
credits unica.it | accessibilità Università degli Studi di Cagliari
C.F.: 80019600925 - P.I.: 00443370929
note legali | privacy

Nascondi la toolbar