|Titolo:||Predictive model for capillary electrophoretic peptide mobility in 2,2,2-trifluoroethanol-water solution|
|Data di pubblicazione:||1998|
|Citazione:||Predictive model for capillary electrophoretic peptide mobility in 2,2,2-trifluoroethanol-water solution / Castagnola M; Rossetti DV; Corda M; Pellegrini M; Misiti F; Olianas A; Giardina B; Messana I. - In: ELECTROPHORESIS. - ISSN 0173-0835. - 19:10(1998), pp. 1728-1732.|
|Abstract:||Using capillary electrophoresis (CE) on a set of 21 peptides with a molecular mass ranging from about 350 to 1850 Da, the Stokes radii at different protonation stages and the acidic dissociation constants in water and in a 2,2,2-trifluoroethanol (TFE) water mixture (30% v/v) were determined. These results permitted us to establish separately the reliability of semiempirical models utilized for the prediction of peptide size and charge at different acidic pH(app) (pH(app) range: 2.00-4.25). The data obtained on size and charge were utilized in order to provide suitable mobility predictions on the basis of the charge-to-size ratio. The best predictive conditions for size and charge were found at the most acidic range of pH(app) studied (2.00-2.25), either in water or a TFE-water mixture, and reliable predictive equations for peptide mobility were established at this pH(app).|
|Tipologia:||1.1 Articolo in rivista|
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