Tecniques

 
  • Plants cultivation (Nicotiana tabacum, Spinacea oleracea, Pisum sativum, Cicer arietinum, Atriplex portulacoides)
  • Preparation and growth of bacterial cell cultures (Escherichia coli, Deinococcus radiodurans, Thermus themophilus, Haloferax volcanii, Synechocystis pcc 6083, Mesorhizobium ciceri)
  • Chemotaxis tests on motile bacteria
  • DNA extraction and purification
  • Purification of membrane proteins from homologous and heterologous systems
  • Fast Protein Liquid Chromatography (FPLC – AKTA systems) for Affinity Chromatography, Ion-Exchange Chromatography, Size Exclusion Chromatography
  • Biochemical methods (SDS-PAGEs, Blue Native-PAGEs, Western Blots, TBE- PAGEs, Agarose gels, BCA Assays, BSA Assays)
  • Mass Spectrometry of proteins
  • Thin Layer Chromatography (TLC) and High Performance Liquid Chromatography (HPLC) of cofactors and amino acids
  • Spectroscopical analysis of cofactor-binding proteins by Absorption Spectroscopy, Fluorescence Spectroscopy, Circular Dichroism Spectroscopy, Infrared Spectroscopy, Thermal Shift Assay
  • Two dimensional and three dimensional crystallisation of membrane proteins
  • Data collection by Electron Crystallography and X-ray Crystallography
  • Preparation and analysis of samples by conventional Room Temperature Electron Microscopy and Cryo-Electron Microscopy
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