Applied Research

 

Implementation of biochemical techniques

We are interested in implementing methods of membrane proteins analysis with the aim to make measurements more comfortable or facilitate the readout of the analysis. Recently, we developed a soft staining procedure aimed at reducing the staining time in native PAGEs and allowing the retention of coloured cofactors in proteins such as photosystems. Similarly, we are also developing a procedure for facilitating the PAGEs analysis of robust auto-assembling S-layer proteins.

 

For details, please see:

Farci D, Kirkpatrick J, Piano D. A new procedure for a fast soft staining of BN-PAGEs on photosynthetic complexes. Electrophoresis, 2016, 38:441-6. doi:10.1002/elps.201600389.

 

 

Biochemical methods’ development for industrial applications

S-layer proteins are important for their potential applications in nanotechnologies. Their are characterised by extreme stability and auto-assembling properties. However, so far their use was limited by their strong tendency to aggregate or crystallise at relatively low concentrations. We developed a procedure which for the first time allows to modulate the auto-assembling by using mild detergents, and to isolate them in high scale. Currently, we are applying these procedures to bacterial S-layers also for the isolation of new types of carotenoids.

For details, please see:

Farci D, Esposito E, El Alaoui E, Piano D. S-layer proteins as a source of carotenoids: isolation of the protein cofactor deinoxanthin from its S-layer protein DR_2577. Food Res Intern, 2016, in press. doi:10.1016/j.foodres.2016.10.003.

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