BL2 (Biosafety Level 2) Virology Laboratories

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Specifications: Laboratories specialized in the growth of laboratory strains and clinical isolates of biosafety level 2 virus.

Equipment availbale: 4 Lamin Flow Cabinets, 4 CO2 Incubators, 1 Inverted Microscope, 4 Refrigerators (+4/-20 °C), 2 Freezers (-20 °C e -80 °C), 1 Centrifuge, 1 Minicentrifuge, 1Spectrophotometers for mw, 1 liquid nitrogen container (-196 °C), 1 Analytical Balance , 1 pHmetro, 1 Thermostatic Bath.

Responsible: Dott.ssa Giuseppina Sanna

Ownership: Department of Biomedical Sciences and Technologies Section of General Microbiology and Virology & Microbial Biotechnologies, University of Cagliari

Main applications:
♦ Growth and titration of biosafety level 2 viruses (BL2): Flaviviruses (YFV, BVDV), Picornaviruses (Entero, Rhino, Hepato, Aphto); Coronaviruses (HCoV); Orthomyxovirus (Influenza); Paramyxoviruses (RSV, Measles); Rhabdoviruses (VSV); Herpesviruses (HSV, HCMV); Iridoviruses (ASFV); Poxviruses (VV), Reoviruses (Blue Tongue).
♦ Evaluation of cytotoxicity (CC50) and of antiviral activity (EC50) of natural and synthesis compounds: cytopathogenic assays (CP), plaque reduction (PR), yield reduction (YR).
♦ Selection of drug-resistance mutants and studies of cross-resistance.