Molecular Biology Laboratory
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Specifications: Lab. specialized in molecular virology and viral enzymology.
Equipment available: 2 Laminar Flow biohazard Hoods, 2 Incubators, 1 Refrigerator (+4/-20 °C) and 1 Freezer (-20 °C), 1 Real-Time PCR instrument, 2 PCR thermocyclers, 1 FPLC, 1 Centrifuge, 2 Refrigerated Centrifuges, 2 Microcentrifuges, 2 Spectrophotometers, 1 Orbital shaker incubator, 1 Hybridiser, 1 Transilluminator, Gel electrophoresis Apparatus for nucleic acids and proteins, 1 Thermostatic Bath, 1 Termoblock.
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Year of acquisition: 2004
Responsibles: Dr. Gabriele Giliberti
Ownership: Department of Biomedical Sciences and Technologies Section of General Microbiology and Virology & Microbial Biotechnologies, University of Cagliari
Main applications:
♦ Real-time PCR analysis of HIV-1 replication in ex vivo target cells.
♦ Nucleic acid extraction, RT-PCR and PCR for amplification and sequencing of wild type and drug-resistant viruses for identification of mutations involved in the acquisition of drug-resistance to nucleoside and non-nucleoside inhibitors of HIV-1 and other RNA viruses.
♦ Cloning and expression in Escherichia coli of viral genes coding for enzymes target of selective antiviral chemotherapy (i.e. RNA polymerases).
♦ Cloning of site-directed mutated genes coding for target enzymes in order to define the role of sites potentially involved in drug-resistance.
♦ Expression and purification of viral wild type enzymes and mutants to be used in in vitro enzymatic assays to evaluate antiviral inhibitors activity.